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KMID : 0358219930200010057
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Korean Journal of Fertility and Sterility
1993 Volume.20 No. 1 p.57 ~ p.64
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Comparison Between a Swim up after Sperm Washing and a Percoll Gradient Technique for Intrauterine Insemination Outcome
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Abstract
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From September 1988 to August 1992, two different methods of preparing human sperm before intrauterine insemination (IUI) were compared using the semen samples of seventy-three infertile couples. The sperms were prepared by a swim-up after sperm
washing
or by a continuous percoll gradient technique.
Fourteen of 35 women conceived during IUI cycles using a sperm washing and swim-up method (40%), and 12 of 38 women conceived during IUI cycles using a percoll gradient technique (31.6%).
Among the group with male infertile etiologic factor only, one of 5 women conceived during sperm washing and swim-up cycles (20%); one of 4 women conceived during percoll gradient cycles (25%). On the contrary, among the group with cervical
factor
only,
six of 1 women conceived during sperm washing and swim-up cycles (60%); Five of 17 women conceived during percoll gradient cycles (29.4%).
It is suggested that sperm separation by sperm washing and swim-up is a useful technique for intrauterine insemination in cervical infertility, and sperm separation in percoll gradient appears to be more valuable for intrauterine insemination of
male
subfertility.
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